To get access to new characters unlock the indicated district, build the listed building, and complete that character's wish list. Characters that have a cost in clams can be unlocked at any time in any district after payment.

Character	How to unlock
Bonnie Swanson	The Swanson House, District 1.
Brain Damaged Horse	Quahog Derby Stables, 120 clams.
Brian Griffin	Quahog Public Library, District 6.
Bruce	The Founding Father, District 2.
Buzz Killington	Barrington Country Club, 250 clams.
Carter Pewterschmidt	Quahog Cinema, District 8.
Chris Griffin	The Griffin House, District 1.
Connie D'Amico	We Heal You Long Time, Unknown District.
Consuela	Consuela's House, 300 clams.
Dr. Elmer Hartman	Quahog Hospital, District 6.
Glenn Quagmire	The Quagmire House, District 1.
Jake Tucker	Church, 50 clams.
Jerome	The Drunken Clam, District 2.
Joe Swanson	James Woods High, District 4.
John Herbert	The Playground, District 4.
Lois Griffin	The Stop'n Shop, District 3.
Meg Griffin	Quahog Oceanland, District 5.
Mort Goldman	Goldman's Pharmacy, District 3.
Seamus	Lighthouse, District 5.
Stewie Griffin	Bob's Funland, District 8.
Tricia Takanawa	PF Chinese, Unknown District.