There are six unlockable characters to be found throughout the game. Some require you to progress through the game, others require a task or challenge. These characters have special traits (or lack thereof) and handle differently than The Player.

Mode	How to unlock
Commander Video	Complete the Bit.Trip arcade game and collect all gold.
Meat Boy	Complete the Meat Boy arcade game and collect all bandages.
Mr. Destructoid	Defeat Dr. Von Buttnick at Mr Destructoid Robot Factory.
Ms. Bates	Defeat Ms. Bates.
Steve from Minecraft	Locate and jump into the pipe in the Gated Community Zone.
Sweat Bomber	Complete the Sweat Bomber mini-game without giving up.