Take about three to five Red Pikmin and go into the corner directly next to Empress Bulblax. Start to throw all the Red Pikmin on it. When it is about to shake them off, whistle your Pikmin back and go into the corner, pressing the C-stick Down, so they will be behind you and therefore will not be crushed. Keep repeating those steps until you defeat Empress Bulblax. This is a good strategy to use to keep most of your Pikmin alive.

This Boss is found in the Frontier Cavern (with larva), Hole of Beasts (without larva), Hole of Heroes (with larva), and Dream Den(without larva). Defeat her by throwing Pikmin at her head. Call your Pikmin back when she flinches.