Sometimes you will find doors that you have to pay to open, one of the items that can be behind those doors is a human heart amulet. Simply open the door and equip the amulet, then go get killed by something. You will be revived and lose the heart amulet and get this achievement. Any door blocking this amulet will always cost 3000 gold (on the first floor anyway), so be sure you have the money for it.