Unlockable - How to Unlock

Hokage (third) - Clear all 30 floors in Second Story
Itachi - Clear all 30 floors in Second Story
Jiraiya - Clear all 100 floors in Story mode
Kabuto - Clear all 100 floors in Story mode
Kisame - Clear all 30 floors in Second Story
Orochimaru - Clear all 100 floors in Story mode
Sasuke - Clear all 100 floors in Story mode
Second Story - Clear all 100 floors in Story mode
Shizune - Clear all 100 floors in Story mode
Tsunade - Clear all 100 floors in Story mode