Fulfill the following criteria to unlock the corresponding characters for Vs. Mode:
Casey Jones: Beat Stage 1 with Raphael
Evil Turtlebot: Beat Stage 3 with any turtle
Hun: Beat Stage 6 with Michaelangelo
Splinter: Beat Dojo Stage 3 with Leonardo
Shredder: Beat Shredder with any turtle
Oroku Saki: Beat Saki with any turtle
Yoshi Hamato: Beat Challenge Mode with any turtle